KeyTec® BIKE, N-His (Sf9)

Quick replication of product information

Used for Developing BIKE Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing BIKE Biochemical Activity Assay or Binding Assay Models

Size:

10 μg

100 μg

Price:

$ 660

CAT.:

P1HI0005S

Delivery:

2-3 weeks

Price:

$ 3433

CAT.:

P1HI0005L

Delivery:

2-3 weeks

KeyTec® BIKE, N-His (Sf9)

10 μg

100 μg

CAT.: P1HI0005S

$ 660 $ 3433

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® BIKE, N-His (Sf9) recombinant protein with N-terminal 6xHis tag was purified by Ni-NTA affinity and followed by SEC chromatography. The SEC-HPLC result showed that the BIKE protein was monomer in the solution. The BIKE protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: Q9NSY1;S38-E345
Tag：N-terminal 6xHis tag
Molecular Weight：36.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：1.0 mg/mL by OD280
Purity：> 90% by SDS-PAGE
Form：Liquid
Formulation：20 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5

Performance

The BIKE activity was detected using ADP-Glo technology. The reaction was performed by incubating the BIKE protein, ATP and substrate at 25 ℃ for 120 min, then reading RLU by Microplate Reader with Luminescence.