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Shanghai VKEY Biotechnologies Co.,Ltd.
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KeyTec® BRAF, N-GST; C-His

Quick replication of product information

KeyTec® BRAF, N-GST; C-His

Size: Used for Developing BRAF Biochemical Activity Assay or Binding Assay Models

Price: $ 661

CAT.: P1HI0234S

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Used for Developing BRAF Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing BRAF Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
Price:
$ 661
CAT.:
P1HI0234S
Delivery:
2-3 weeks
Price:
$ 5291
CAT.:
P1HI0234L
Delivery:
2-3 weeks
KeyTec® BRAF, N-GST; C-His
10 μg
10 μg
100 μg
CAT.: P1HI0234S
$ 661 $ 5291
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® BRAF, N-GST; C-His recombinant protein with N-terminal GST tag and C-terminal 6x His tag was purified by Ni-NTA affinity and followed by GST affinity chromatography. The BRAF protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: P15056, E433-R726
Tag： N-terminal GST tag, C-terminal 6x His tag
Molecular Weight： 61.3 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 1.00 mg/mL by OD280
Purity： > 80% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5

Performance

P1HI0234_2.1.png
P1HI0234_2.2.png
The BRAF activity was detected using ADP-Glo technology. The reaction was performed by incubating the BRAF protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
P1HI0234_2.3.png

Protocols

COA_P1HI0234_G140223021_EN.pdf
COA_P1HI0234_G140223021_EN.pdf Download

Components

CAT. Description Size
P1HI0234S KeyTec® BRAF, N-GST; C-His 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® BRAF, N-GST; C-His recombinant protein with N-terminal GST tag and C-terminal 6x His tag was purified by Ni-NTA affinity and followed by GST affinity chromatography. The BRAF protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: P15056, E433-R726
Tag： N-terminal GST tag, C-terminal 6x His tag
Molecular Weight： 61.3 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 1.00 mg/mL by OD280
Purity： > 80% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5

Performance

P1HI0234_2.1.png
P1HI0234_2.2.png
The BRAF activity was detected using ADP-Glo technology. The reaction was performed by incubating the BRAF protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
P1HI0234_2.3.png

Protocols

COA_P1HI0234_G140223021_EN.pdf
COA_P1HI0234_G140223021_EN.pdf Download

Components

CAT. Description Size
P1HI0234L KeyTec® BRAF, N-GST; C-His 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

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