KeyTec® BTK[C481S], N-GST

Quick replication of product information

Used for Developing BTK[C481R] Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing BTK[C481R] Biochemical Activity Assay or Binding Assay Models

Size:

10 μg

100 μg

Price:

$ 945

CAT.:

P1HI0008S

Delivery:

2-3 weeks

Price:

$ 5291

CAT.:

P1HI0008L

Delivery:

2-3 weeks

KeyTec® BTK[C481S], N-GST

10 μg

100 μg

CAT.: P1HI0008S

$ 945 $ 5291

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® BTK[C481S], N-GST recombinant protein with N-terminal GST tag was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the BTK[C481S] protein was dimer in the solution. The BTK[C481S] protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: Q06187, A2-S659, C481S
Tag：N-terminal GST tag
Molecular Weight：101.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.41 mg/mL by OD280
Purity：> 80% by SDS-PAGE
Form：Liquid
Formulation：50 mM Hepes, 150 mM NaCl, 20 mM Reduced Glu, 1 mM DTT, 10% glycerol, pH 7.5

Performance

The BTK[C481S] activity was detected using TR-FRET technology. The reaction was performed by incubating the BTK[C481S] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.