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Shanghai VKEY Biotechnologies Co.,Ltd.
Shanghai VKEY Biotechnologies Co.,Ltd.
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KeyTec® CaMK2a, N-GST

Quick replication of product information

KeyTec® CaMK2a, N-GST

Size: Used for Developing CaMK2α Biochemical Activity Assay or Binding Assay Models

Price: $ 660

CAT.: P1HI0014S

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Used for Developing CaMK2α Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing CaMK2α Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
Price:
$ 660
CAT.:
P1HI0014S
Delivery:
2-3 weeks
Price:
$ 3433
CAT.:
P1HI0014L
Delivery:
2-3 weeks
KeyTec® CaMK2a, N-GST
10 μg
10 μg
100 μg
CAT.: P1HI0014S
$ 660 $ 3433
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® CaMK2α, N-GST recombinant protein with N-terminal GST tag was purified by GST affinity chromatography. The CaMK2α protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: Q9UQM7-1, M1-H478(end)
Tag： N-terminal GST tag
Molecular Weight： 80.7 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.43 mg/mL by OD280
Purity： > 90% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH 7.5

Performance

P1HI0014_2.1.png
P1HI0014_2.2.png
The CaMK2α activity was detected using TR-FRET technology. The reaction was performed by incubating the CaMK2α protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0014_2.3.png

Protocols

COA_P1HI0014_G130613011_EN.pdf
COA_P1HI0014_G130613011_EN.pdf Download

Components

CAT. Description Size
P1HI0014S KeyTec® CaMK2a, N-GST 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® CaMK2α, N-GST recombinant protein with N-terminal GST tag was purified by GST affinity chromatography. The CaMK2α protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: Q9UQM7-1, M1-H478(end)
Tag： N-terminal GST tag
Molecular Weight： 80.7 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.43 mg/mL by OD280
Purity： > 90% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH 7.5

Performance

P1HI0014_2.1.png
P1HI0014_2.2.png
The CaMK2α activity was detected using TR-FRET technology. The reaction was performed by incubating the CaMK2α protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0014_2.3.png

Protocols

COA_P1HI0014_G130613011_EN.pdf
COA_P1HI0014_G130613011_EN.pdf Download

Components

CAT. Description Size
P1HI0014L KeyTec® CaMK2a, N-GST 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

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