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Shanghai VKEY Biotechnologies Co.,Ltd.
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KeyTec® cAMP TR-FRET KIT

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KeyTec® cAMP TR-FRET KIT

Size: Competitive Model TR-FRET Assay for Quantitative Measurement of cAMP in cell lysates

Price: $ 912

CAT.: A010101

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Competitive Model TR-FRET Assay for Quantitative Measurement of cAMP in cell lysates


• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Range: Based on competitive immunoassay model, Wide linear range
• Used to detect changes in cAMP accumulation, when Gs and Gi signaling pathways are activated or inhibited

Competitive Model TR-FRET Assay for Quantitative Measurement of cAMP in cell lysates


• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Range: Based on competitive immunoassay model, Wide linear range
• Used to detect changes in cAMP accumulation, when Gs and Gi signaling pathways are activated or inhibited

Competitive Model TR-FRET Assay for Quantitative Measurement of cAMP in cell lysates


• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Range: Based on competitive immunoassay model, Wide linear range
• Used to detect changes in cAMP accumulation, when Gs and Gi signaling pathways are activated or inhibited

Competitive Model TR-FRET Assay for Quantitative Measurement of cAMP in cell lysates


• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Range: Based on competitive immunoassay model, Wide linear range
• Used to detect changes in cAMP accumulation, when Gs and Gi signaling pathways are activated or inhibited

Size:
1000 tests
10000 tests
50000 tests
100000 tests
Price:
$ 912
CAT.:
A010101
Delivery:
Available in Stock
Price:
$ 2840
CAT.:
A010012
Delivery:
Available in Stock
Price:
$ 12476
CAT.:
A010013
Delivery:
Available in Stock
Price:
$ 23308
CAT.:
A010014
Delivery:
Available in Stock
KeyTec® cAMP TR-FRET KIT
1000 tests
1000 tests
10000 tests
50000 tests
100000 tests
CAT.: A010101
$ 912 $ 2840 $ 12476 $ 23308
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® cAMP TR-FRET KIT designed for quantitative measurement of adenosine 3',5'-cyclic adenosine monophosphate (cAMP) in cell lysates. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The detection principle is based on TR-FRET technology. Streptavidin and anti-cAMP antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, Streptavidin-Solar Eu form a complex with Biotin–cAMP referred to as the cAMP tracer. When cAMP tracer binds to mAb anti-cAMP–LA, excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native cAMP produced by cells or unlabeled cAMP (standard curve) competes with the cAMP tracer for binding to anti-cAMP antibodies. The specific signal is inversely proportional to the concentration of cAMP in the standard or sample.
EN_A010101_2.1.png

Performance

Protocols

Components

CAT. Description Size
A010101 KeyTec® cAMP TR-FRET KIT 1000 tests
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
• 1 × 50 µL mAb anti-cAMP-LA (100X)
• 1 × 25 μL Biotin-cAMP (200X)
• 1 × 100 µL cAMP Standard Solution (50 μM)
• 1 × 25 μL Streptavidin-Solar Eu (200X)
• 2 × 1 mL Detection Buffer (5X)

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -60℃ or below

-60℃ or below

Overview

KeyTec® cAMP TR-FRET KIT designed for quantitative measurement of adenosine 3',5'-cyclic adenosine monophosphate (cAMP) in cell lysates. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The detection principle is based on TR-FRET technology. Streptavidin and anti-cAMP antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, Streptavidin-Solar Eu form a complex with Biotin–cAMP referred to as the cAMP tracer. When cAMP tracer binds to mAb anti-cAMP–LA, excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native cAMP produced by cells or unlabeled cAMP (standard curve) competes with the cAMP tracer for binding to anti-cAMP antibodies. The specific signal is inversely proportional to the concentration of cAMP in the standard or sample.
EN_A010101_2.1.png

Performance

Protocols

Components

CAT. Description Size
A010012 KeyTec® cAMP TR-FRET KIT 10000 tests
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
• 1 × 500 µL mAb anti-cAMP-LA (100X)
• 1 × 250 μL Biotin-cAMP (200X)
• 2 vials cAMP Standard Solution (50 μM)
• 1 × 250 μL Streptavidin-Solar Eu (200X)
• 1 × 20 mL Detection Buffer (5X)

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -60℃ or below

-60℃ or below

Overview

KeyTec® cAMP TR-FRET KIT designed for quantitative measurement of adenosine 3',5'-cyclic adenosine monophosphate (cAMP) in cell lysates. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The detection principle is based on TR-FRET technology. Streptavidin and anti-cAMP antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, Streptavidin-Solar Eu form a complex with Biotin–cAMP referred to as the cAMP tracer. When cAMP tracer binds to mAb anti-cAMP–LA, excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native cAMP produced by cells or unlabeled cAMP (standard curve) competes with the cAMP tracer for binding to anti-cAMP antibodies. The specific signal is inversely proportional to the concentration of cAMP in the standard or sample.
EN_A010101_2.1.png

Performance

Protocols

Components

CAT. Description Size
A010013 KeyTec® cAMP TR-FRET KIT 50000 tests
Customized sizes

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -60℃ or below

-60℃ or below

Overview

KeyTec® cAMP TR-FRET KIT designed for quantitative measurement of adenosine 3',5'-cyclic adenosine monophosphate (cAMP) in cell lysates. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The detection principle is based on TR-FRET technology. Streptavidin and anti-cAMP antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, Streptavidin-Solar Eu form a complex with Biotin–cAMP referred to as the cAMP tracer. When cAMP tracer binds to mAb anti-cAMP–LA, excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native cAMP produced by cells or unlabeled cAMP (standard curve) competes with the cAMP tracer for binding to anti-cAMP antibodies. The specific signal is inversely proportional to the concentration of cAMP in the standard or sample.
EN_A010101_2.1.png

Performance

Protocols

Components

CAT. Description Size
A010014 KeyTec® cAMP TR-FRET KIT 100000 tests
Customized sizes

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -60℃ or below

-60℃ or below

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