KeyTec® CDK12/Cyclin K, N-GST

Quick replication of product information

Used for Developing CDK12/Cyclin K Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing CDK12/Cyclin K Biochemical Activity Assay or Binding Assay Models

Size:

10 μg

100 μg

Price:

$ 660

CAT.:

P1HI0102S

Delivery:

2-3 weeks

Price:

$ 3433

CAT.:

P1HI0102L

Delivery:

2-3 weeks

KeyTec® CDK12/Cyclin K, N-GST

10 μg

100 μg

CAT.: P1HI0102S

$ 660 $ 3433

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® CDK12/Cyclin K, N-GST recombinant protein was co-expressed recombinant human CDK12 with N-terminal GST tag + TEV cleavage site and Cyclin K protein with N-terminal Flag tag and C-terminal 6xHis tag. The complex was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the CDK12/Cyclin K protein was polymer in the solution. The CDK12/ Cyclin K protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK12:Q9NYV4, Q696-S1082; Cyclin K: O75909, M1-S300
Tag： CDK12:N-terminal GST tag; Cyclin K: N-terminal-Flag, C-His
Molecular Weight： CDK12:61.7 kDa; Cyclin K: 37.2 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.58 mg/mL by OD280
Purity：> 85% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 20 mM reduced glutathione , 1 mM DTT, 0.05% Brij35, 10% glycerol, pH7.5

Performance

The CDK12/Cyclin K activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK12/ Cyclin K protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.