KeyTec® CDK13/Cyclin K, N-GST/N-FLAG; C-His

Quick replication of product information

Used for Developing CDK13/Cyclin K Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing CDK13/Cyclin K Biochemical Activity Assay or Binding Assay Models

Size:

10 μg

100 μg

Price:

$ 945

CAT.:

P1HI0018S

Delivery:

2-3 weeks

Price:

$ 5291

CAT.:

P1HI0018L

Delivery:

2-3 weeks

KeyTec® CDK13/Cyclin K, N-GST/N-FLAG; C-His

10 μg

100 μg

CAT.: P1HI0018S

$ 945 $ 5291

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® CDK13/Cyclin K, N-GST/ N-FLAG; C-His recombinant protein was co-expressed recombinant human CDK13 with N-terminal GST tag and Cyclin K protein with N-terminal GST tag and C-terminal 6xHis tag. The complex was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the CDK13/Cyclin K protein was polymer in the solution. The CDK13/Cyclin K protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK13: Q14004, Q673-S1054; Cyclin K: O75909, M1-S300
Tag： CDK13: N-terminal GST tag; Cyclin K: N-terminal-Flag, C-His
Molecular Weight： CDK13: 70.3 kDa; Cyclin K: 37.2 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.33 mg/mL by OD280
Purity：> 85% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH 7.5

Performance

The CDK13/Cyclin K activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK13/Cyclin K protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.