KeyTec® CDK1/CycA2, N-GST/N-GST;C-His

KeyTec® CDK1/CycA2, N-GST/N-GST;C-His recombinant protein was co-expressed recombinant human CDK1 with N-terminal GST tag, CycA2 protein with N-terminal GST tag and C- terminal 8x His tag. Recombinant human CDK1/CycA2 protein was purified by GST affinity and followed by SEC chromatography. The CDK1/CycA2 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK1: P06493-1, M1-M297(end); CycA2: P20248, E174-L432(end)
Tag： CDK1: N-terminal GST tag; CycA2: N-terminal GST tag, C-terminal 8x His tag
Molecular Weight： CDK1: 60.7 kDa; CycA2: 56.8 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.99 mg/mL by OD280
Purity： >95% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5