Shanghai VKEY Biotechnologies Co.,Ltd.
Shanghai VKEY Biotechnologies Co.,Ltd.
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KeyTec® CDK2/CycA2, N-GST/C-His

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KeyTec® CDK2/CycA2, N-GST/C-His

Size: Used for Developing CDK2/CycA2 Biochemical Activity Assay or Binding Assay Models

Price: $ 660

CAT.: P1HI0107S

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Used for Developing CDK2/CycA2 Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing CDK2/CycA2 Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
Price:
$ 660
CAT.:
P1HI0107S
Delivery:
2-3 weeks
Price:
$ 3433
CAT.:
P1HI0107L
Delivery:
2-3 weeks
KeyTec® CDK2/CycA2, N-GST/C-His
10 μg
10 μg
100 μg
CAT.: P1HI0107S
$ 660 $ 3433
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® CDK2/CycA2, N-GST/C-His recombinant protein was co-expressed recombinant human CDK2 with N-terminal GST tag, CycA2 protein with C- terminal 8x His tag. Recombinant human CDK2/CycA2 protein was purified by GST affinity. The CDK2/CycA2 protein showed high activity in ADP-Glo assay.
AA Sequences: Uniprot: CDK2: P24941-1, M1-L298 (end); CycA2: P20248, E174-L432(end)
Tag: CDK2: N-terminal GST tag; CycA2: C-terminal 8x His tag
Molecular Weight: CDK2: 60.6 kDa; CycA2: 31.1 kDa
Species: Human
Expression Host: Sf9
Protein Concentration: 0.42 mg/mL by OD280
Purity: >95% by SDS-PAGE
Form: Liquid
Formulation: 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5

Performance

P1HI0107_2.1.png
P1HI0107_2.2.png
The CDK2/CycA2 activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK2/CycA2 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
P1HI0107_2.3.png

Protocols

Components

CAT. Description Size
P1HI0107S KeyTec® CDK2/CycA2, N-GST/C-His 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® CDK2/CycA2, N-GST/C-His recombinant protein was co-expressed recombinant human CDK2 with N-terminal GST tag, CycA2 protein with C- terminal 8x His tag. Recombinant human CDK2/CycA2 protein was purified by GST affinity. The CDK2/CycA2 protein showed high activity in ADP-Glo assay.
AA Sequences: Uniprot: CDK2: P24941-1, M1-L298 (end); CycA2: P20248, E174-L432(end)
Tag: CDK2: N-terminal GST tag; CycA2: C-terminal 8x His tag
Molecular Weight: CDK2: 60.6 kDa; CycA2: 31.1 kDa
Species: Human
Expression Host: Sf9
Protein Concentration: 0.42 mg/mL by OD280
Purity: >95% by SDS-PAGE
Form: Liquid
Formulation: 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5

Performance

P1HI0107_2.1.png
P1HI0107_2.2.png
The CDK2/CycA2 activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK2/CycA2 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
P1HI0107_2.3.png

Protocols

Components

CAT. Description Size
P1HI0107L KeyTec® CDK2/CycA2, N-GST/C-His 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

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