KeyTec® CDK3/CycE1, N-GST/N-His

Quick replication of product information

Used for Developing CDK3/CycE1 Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing CDK3/CycE1 Biochemical Activity Assay or Binding Assay Models

Size:

10 μg

100 μg

Price:

$ 660

CAT.:

P1HI0022S

Delivery:

2-3 weeks

Price:

$ 3433

CAT.:

P1HI0022L

Delivery:

2-3 weeks

KeyTec® CDK3/CycE1, N-GST/N-His

10 μg

100 μg

CAT.: P1HI0022S

$ 660 $ 3433

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® CDK3/CycE1, N-GST/N-His recombinant protein was co-expressed recombinant human CDK3 with N-terminal GST tag and CycE1 protein with N-terminal 6xHis tag. The complex was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the CDK3/ CycE1 protein was polymer in the solution. The CDK3/CycE1 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK3: Q00526, M1-H305(end); CycE1: P24864,M1-A410(end)
Tag： CDK3: N-terminal GST tag; CycE1: N-terminal 6xHis Tag
Molecular Weight： CDK3: 61.7 kDa; CycE1: 48.1 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 1.27 mg/mL by OD280
Purity： > 85% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 20 mM reduced glutathione , 1 mM DTT, 0.05% Brij35, 10% glycerol, pH 7.5

Performance

The CDK3/CycE1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the CDK3/CycE1 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.