KeyTec® CDK4/CycD1, N-GST/N-GST;C-His

KeyTec® CDK4/CycD1, N-GST/N-GST;C-His recombinant protein was co-expressed recombinant human CDK4 with N-terminal GST tag and CycD1 with N-terminal GST tag and C-terminal 6x His tag. The complex was purified by GST affinity chromatography. The CDK4/CycD1 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CDK4:P11802-1, M1-E303(end); CycD1:P24385, M1-I295(end)
Tag： CDK4:N-terminal GST tag; CycD1:N-terminal GST tag, C-terminal 6x His tag
Molecular Weight： CDK4:59.6 kDa; CycD1:60.5 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.68 mg/mL by OD280
Purity： > 90% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 20 mM reduced glutathione , 1 mM DTT, 0.1mM PMSF, 0.05% Brij35, 10% glycerol, pH7.5