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KeyTec® CK2α1/CK2β, N-GST/N-His

Quick replication of product information

KeyTec® CK2α1/CK2β, N-GST/N-His

Size: Used for Developing CK2α1/CK2β Biochemical Activity Assay or Binding Assay Models

Price: $ 660

CAT.: P1HI0027S

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Used for Developing CK2α1/CK2β Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing CK2α1/CK2β Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
Price:
$ 660
CAT.:
P1HI0027S
Delivery:
2-3 weeks
Price:
$ 3433
CAT.:
P1HI0027L
Delivery:
2-3 weeks
KeyTec® CK2α1/CK2β, N-GST/N-His
10 μg
10 μg
100 μg
CAT.: P1HI0027S
$ 660 $ 3433
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® CK2α1/CK2β, N-GST/N-His recombinant protein was co-expressed recombinant human CK2α1 with N-terminal GST tag and CK2β protein with N-terminal 6xHis tag. The complex was purified by GST affinity chromatography. The CK2α1/CK2β protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CK2α1: P68400-1, M1-Q391(end); CK2β: N0E4C7, M1-R215(end)
Tag： CK2α1: N-terminal GST tag; CK2β: N-terminal 6x His tag
Molecular Weight： CK2α1: 71.8 kDa; CK2β: 25.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.49 mg/mL by OD280
Purity：> 85% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 300 mM NaCl, 1mM DTT, 10% glycerol, pH7.5

Performance

P1HI0027_2.1.png
P1HI0027_2.2.png
The CK2α1/CK2β activity was detected using ADP-Glo technology. The reaction was performed by incubating the CK2α1/CK2β protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
P1HI0027_2.3.png

Protocols

COA_P1HI0027_K130214011_EN.pdf
COA_P1HI0027_K130214011_EN.pdf Download

Components

CAT. Description Size
P1HI0027S KeyTec® CK2α1/CK2β, N-GST/N-His 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® CK2α1/CK2β, N-GST/N-His recombinant protein was co-expressed recombinant human CK2α1 with N-terminal GST tag and CK2β protein with N-terminal 6xHis tag. The complex was purified by GST affinity chromatography. The CK2α1/CK2β protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CK2α1: P68400-1, M1-Q391(end); CK2β: N0E4C7, M1-R215(end)
Tag： CK2α1: N-terminal GST tag; CK2β: N-terminal 6x His tag
Molecular Weight： CK2α1: 71.8 kDa; CK2β: 25.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.49 mg/mL by OD280
Purity：> 85% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 300 mM NaCl, 1mM DTT, 10% glycerol, pH7.5

Performance

P1HI0027_2.1.png
P1HI0027_2.2.png
The CK2α1/CK2β activity was detected using ADP-Glo technology. The reaction was performed by incubating the CK2α1/CK2β protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
P1HI0027_2.3.png

Protocols

COA_P1HI0027_K130214011_EN.pdf
COA_P1HI0027_K130214011_EN.pdf Download

Components

CAT. Description Size
P1HI0027L KeyTec® CK2α1/CK2β, N-GST/N-His 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

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