KeyTec® CK2α2[C336S]/CK2β, N-GST/N-His

Quick replication of product information

Used for Developing CK2α2[C336S]/CK2β Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing CK2α2[C336S]/CK2β Biochemical Activity Assay or Binding Assay Models

Size:

10 μg

100 μg

Price:

$ 660

CAT.:

P1HI0029S

Delivery:

2-3 weeks

Price:

$ 3433

CAT.:

P1HI0029L

Delivery:

2-3 weeks

KeyTec® CK2α2[C336S]/CK2β, N-GST/N-His

10 μg

100 μg

CAT.: P1HI0029S

$ 660 $ 3433

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® CK2α2[C336S]/CK2β, N-GST/N-His recombinant protein was co-expressed recombinant human CK2α2[C336S] with N-terminal GST tag and CK2β protein with N-terminal 6xHis tag. The complex was purified by Ni-NTA affinity chromatography. The CK2α2[C336S]/CK2β protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: CK2α2: P19784, M1-R350(end),C336S; CK2β: N0E4C7, M1-R215(end)
Tag： CK2α2[C336S]: N-terminal GST tag; CK2β: N-terminal 6x His tag
Molecular Weight： CK2α2[C336S]: 67.8 kDa; CK2β: 25.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.83 mg/mL by OD280
Purity：> 90% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5

Performance

The CK2α2[C336S]/CK2β activity was detected using ADP-Glo technology. The reaction was performed by incubating the CK2α2[C336S]/CK2β protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.

Protocols

COA_P1HI0029_G130706011_EN.pdf Download

Components

CAT.	Description	Size
P1HI0029S	KeyTec® CK2α2[C336S]/CK2β, N-GST/N-His	10 μg

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-80 ℃

Overview

Performance

Protocols

COA_P1HI0029_G130706011_EN.pdf Download

Components

CAT.	Description	Size
P1HI0029L	KeyTec® CK2α2[C336S]/CK2β, N-GST/N-His	100 μg

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-80 ℃

KeyTec® CK2α2[C336S]/CK2β, N-GST/N-His

Overview

Performance

Protocols

Components

Notices

Overview

Performance

Protocols

Components

Notices

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