(+86)-021-5835-6939 marketing@vkeybio.com
EnglishEnglish
Shanghai VKEY Biotechnologies Co.,Ltd.
Shanghai VKEY Biotechnologies Co.,Ltd.
Email Us

KeyTec® EGFR, N-His

Quick replication of product information

KeyTec® EGFR, N-His

Size: Used for Developing EGFR Biochemical Activity Assay or Binding Assay Models

Price: $ 660

CAT.: P1HI0032S

激酶_1.1.jpg
激酶_1.2.jpg
激酶_1.1.jpg
激酶_1.1.jpg
激酶_1.1.jpg
激酶_1.2.jpg
激酶_1.1.jpg
激酶_1.1.jpg
Used for Developing EGFR Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing EGFR Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
Price:
$ 660
CAT.:
P1HI0032S
Delivery:
2-3 weeks
Price:
$ 3990
CAT.:
P1HI0032L
Delivery:
2-3 weeks
KeyTec® EGFR, N-His
10 μg
10 μg
100 μg
CAT.: P1HI0032S
$ 660 $ 3990
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® EGFR, N-His recombinant protein with N-terminal 6xHis tag + TEV cleavage site was purified by Ni-NTA affinity chromatography. The EGFR protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P00533-1; H672-Y998
Tag： N-terminal 6x His tag
Molecular Weight： 39.5 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.55 mg/mL by OD280
Purity：> 80% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Hepes, 300 mM NaCl, 1 mM DTT, 10% Glycerol, pH7.5

Performance

P1HI0032_2.1.png
P1HI0032_2.2.png
The EGFR activity was detected using TR-FRET technology. The reaction was performed by incubating the EGFR protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0032_2.3.png

Protocols

COA_P1HI0032_K121222021_EN.pdf
COA_P1HI0032_K121222021_EN.pdf Download

Components

CAT. Description Size
P1HI0032S KeyTec® EGFR, N-His 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® EGFR, N-His recombinant protein with N-terminal 6xHis tag + TEV cleavage site was purified by Ni-NTA affinity chromatography. The EGFR protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P00533-1; H672-Y998
Tag： N-terminal 6x His tag
Molecular Weight： 39.5 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.55 mg/mL by OD280
Purity：> 80% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Hepes, 300 mM NaCl, 1 mM DTT, 10% Glycerol, pH7.5

Performance

P1HI0032_2.1.png
P1HI0032_2.2.png
The EGFR activity was detected using TR-FRET technology. The reaction was performed by incubating the EGFR protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0032_2.3.png

Protocols

COA_P1HI0032_K121222021_EN.pdf
COA_P1HI0032_K121222021_EN.pdf Download

Components

CAT. Description Size
P1HI0032L KeyTec® EGFR, N-His 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Related Kinase

KeyTec® CDK2/CyclinE2, N-GST/N-GST KeyTec® CDK2/CyclinE2, N-GST/N-GST
KeyTec® FGFR3 [V553M], N-GST KeyTec® FGFR3 [V553M], N-GST
KeyTec® EGFR[L861Q], N-GST KeyTec® EGFR[L861Q], N-GST
KeyTec® CDK9/Cyclin K, N-GST/N-Flag;C-His KeyTec® CDK9/Cyclin K, N-GST/N-Flag;C-His
KeyTec® EGFR[L858R/T790M], N-GST KeyTec® EGFR[L858R/T790M], N-GST
KeyTec® TYK2(JH1), N-GST KeyTec® TYK2(JH1), N-GST
KeyTec® FLT3-ITD, N-GST; C-His KeyTec® FLT3-ITD, N-GST; C-His
KeyTec® BUB1/BUB3, N-GST; C-Flag/C-Flag KeyTec® BUB1/BUB3, N-GST; C-Flag/C-Flag