Shanghai VKEY Biotechnologies Co.,Ltd.
Shanghai VKEY Biotechnologies Co.,Ltd.
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KeyTec® EGFR[d746-750/T790M], N-GST

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KeyTec® EGFR[d746-750/T790M], N-GST

Size: Used for Developing EGFR[d746-750/T790M] Biochemical Activity Assay or Binding Assay Models

Price: $ 660

CAT.: P1HI0036S

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Used for Developing EGFR[d746-750/T790M] Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing EGFR[d746-750/T790M] Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing EGFR[d746-750/T790M] Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing EGFR[d746-750/T790M] Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
10 μg
100 μg
Price:
$ 660
CAT.:
P1HI0036S
Delivery:
2-3 weeks
Price:
$ 5291
CAT.:
P1HI0036L
Delivery:
2-3 weeks
Price:
$ 661
CAT.:
P1HI0211S
Delivery:
2-3 weeks
Price:
$ 5291
CAT.:
P1HI0211L
Delivery:
2-3 weeks
KeyTec® EGFR[d746-750/T790M], N-GST
10 μg
10 μg
100 μg
10 μg
100 μg
CAT.: P1HI0036S
$ 660 $ 5291 $ 661 $ 5291
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® EGFR[d746-750/T790M], N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity chromatography. The EGFR[d746-750/T790M] protein showed high activity in TR-FRET assay.
AA Sequences: Uniprot: P00533-1; R669-A1210(end), d746-750, T790M
Tag: N-terminal GST tag
Molecular Weight: 87.2 kDa
Species: Human
Expression Host: Sf9
Protein Concentration: 0.21 mg/mL by OD280
Purity:> 60% by SDS-PAGE
Form: Liquid
Formulation: 50 mM Hepes, 150 mM NaCl, 20 mM Reduced Glu, 1 mM DTT, 10% Glycerol, pH7.5

Performance

P1HI0036_2.1.png
P1HI0036_2.2.png
The EGFR[d746-750/T790M] activity was detected using TR-FRET technology. The reaction was performed by incubating the EGFR[d746-750/T790M] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0036_2.3.png

Protocols

Components

CAT. Description Size
P1HI0036S KeyTec® EGFR[d746-750/T790M], N-GST 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® EGFR[d746-750/T790M], N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity chromatography. The EGFR[d746-750/T790M] protein showed high activity in TR-FRET assay.
AA Sequences: Uniprot: P00533-1; R669-A1210(end), d746-750, T790M
Tag: N-terminal GST tag
Molecular Weight: 87.2 kDa
Species: Human
Expression Host: Sf9
Protein Concentration: 0.21 mg/mL by OD280
Purity:> 60% by SDS-PAGE
Form: Liquid
Formulation: 50 mM Hepes, 150 mM NaCl, 20 mM Reduced Glu, 1 mM DTT, 10% Glycerol, pH7.5

Performance

P1HI0036_2.1.png
P1HI0036_2.2.png
The EGFR[d746-750/T790M] activity was detected using TR-FRET technology. The reaction was performed by incubating the EGFR[d746-750/T790M] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0036_2.3.png

Protocols

Components

CAT. Description Size
P1HI0036L KeyTec® EGFR[d746-750/T790M], N-GST 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

Please contact sales for details

Performance

Components

CAT. Description Size
P1HI0211S KeyTec® EGFR[d746-750/T790M], N-GST 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

Please contact sales for details

Performance

Components

CAT. Description Size
P1HI0211L KeyTec® EGFR[d746-750/T790M], N-GST 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

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