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KeyTec® EGFR[T790M/C797S/L858R], N-GST H672-Y998

Quick replication of product information

KeyTec® EGFR[T790M/C797S/L858R], N-GST H672-Y998

Size: Used for Developing EGFR[T790M/C797S/L858R] Biochemical Activity Assay or Binding Assay Models

Price: $ 660

CAT.: P1HI0124S

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Used for Developing EGFR[T790M/C797S/L858R] Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing EGFR[T790M/C797S/L858R] Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
Price:
$ 660
CAT.:
P1HI0124S
Delivery:
2-3 weeks
Price:
$ 3990
CAT.:
P1HI0124L
Delivery:
2-3 weeks
KeyTec® EGFR[T790M/C797S/L858R], N-GST H672-Y998
10 μg
10 μg
100 μg
CAT.: P1HI0124S
$ 660 $ 3990
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® EGFR[T790M/C797S/L858R], N-GST H672-Y998 recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity chromatography. The EGFR[T790M/C797S/L858R] protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P00533-1; H672-Y998, T790M, C797S, L858R
Tag： N-terminal GST tag
Molecular Weight： 64.1 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.78 mg/mL by OD280
Purity：>40% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 300 mM NaCl, 10 mM reduced glutathione, 1 mM DTT, 10% glycerol, pH8.0

Performance

P1HI0124_2.1.png
P1HI0124_2.2.png
The EGFR[T790M/C797S/L858R] activity was detected using TR-FRET technology. The reaction was performed by incubating the EGFR[T790M/C797S/L858R] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0124_2.3.png

Protocols

COA_P1HI0124_G130226011_EN.pdf
COA_P1HI0124_G130226011_EN.pdf Download

Components

CAT. Description Size
P1HI0124S KeyTec® EGFR[T790M/C797S/L858R], N-GST H672-Y998 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® EGFR[T790M/C797S/L858R], N-GST H672-Y998 recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity chromatography. The EGFR[T790M/C797S/L858R] protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P00533-1; H672-Y998, T790M, C797S, L858R
Tag： N-terminal GST tag
Molecular Weight： 64.1 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.78 mg/mL by OD280
Purity：>40% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 300 mM NaCl, 10 mM reduced glutathione, 1 mM DTT, 10% glycerol, pH8.0

Performance

P1HI0124_2.1.png
P1HI0124_2.2.png
The EGFR[T790M/C797S/L858R] activity was detected using TR-FRET technology. The reaction was performed by incubating the EGFR[T790M/C797S/L858R] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0124_2.3.png

Protocols

COA_P1HI0124_G130226011_EN.pdf
COA_P1HI0124_G130226011_EN.pdf Download

Components

CAT. Description Size
P1HI0124L KeyTec® EGFR[T790M/C797S/L858R], N-GST H672-Y998 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

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