KeyTec® Enhanced Luciferase Detection Kit (no DTT)

Quick replication of product information

Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: Wider linear range and robust luminescence signal and without DTT
• The luminescence signal is not only high but also robust, with a half-life of approximately 1 h. It is suitable for most assay formats

Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays

Size:

10 mL

2*50 mL

2*250 mL

Price:

$ 113

CAT.:

A2000600N

Delivery:

Available in Stock

Price:

$ 705

CAT.:

A2000601N

Delivery:

Available in Stock

Price:

$ 2340

CAT.:

A2000602N

Delivery:

Available in Stock

KeyTec® Enhanced Luciferase Detection Kit (no DTT)

10 mL

2*50 mL

2*250 mL

CAT.: A2000600N

$ 113 $ 705 $ 2340

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® Enhanced Luciferase Detection Kit (no DTT) is designed for the sensitive and robust detection of Firefly luciferase reporter gene assays without Dithiothreitol. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with Firefly luciferase released by cells, generating a sensitive and robust luminescence signal. The process provides a highly sensitive, robust, and homogeneous assay for the detection of Firefly luciferase reporter gene expression in mammalian cells.

Performance

Use KeyTec® Enhanced Luciferase Detection Kit (no DTT) to detect the overexpression of Firefly luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).

Protocols

A2000602N_KeyTec® Enhanced Luciferase Detection Kit (no DTT)_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A2000600N	KeyTec® Enhanced Luciferase Detection Kit (no DTT)	10 mL
Each kit contains sufficient reagents to perform 100 tests of 100 μL/well. The kit contains the following components: • 1 × 200 μL KeyTec® Enhanced Luciferase Substrate 50X (no DTT) • 1 × 10 mL KeyTec® Enhanced Luciferase Cell lysis buffer (no DTT)

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-40 ℃

Overview

Performance

Protocols

A2000602N_KeyTec® Enhanced Luciferase Detection Kit (no DTT)_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A2000601N	KeyTec® Enhanced Luciferase Detection Kit (no DTT)	2*50 mL
Each kit contains sufficient reagents to perform 1,000 tests of 100 μL/well. The kit contains the following components: • 2 × 1 mL KeyTec® Enhanced Luciferase Substrate 50X (no DTT) • 2 × 50 mL KeyTec® Enhanced Luciferase Cell lysis buffer (no DTT)

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-40 ℃

Overview

Performance

Protocols

A2000602N_KeyTec® Enhanced Luciferase Detection Kit (no DTT)_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A2000602N	KeyTec® Enhanced Luciferase Detection Kit (no DTT)	2*250 mL
Each kit contains sufficient reagents to perform 5,000 tests of 100 μL/well. The kit contains the following components: • 5 × 2 mL KeyTec® Enhanced Luciferase Substrate 50X (no DTT) • 2 × 250 mL KeyTec® Enhanced Luciferase Cell lysis buffer (no DTT)