KeyTec® EPHA4, N-GST

Quick replication of product information

Used for Developing EPHA4 Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing EPHA4 Biochemical Activity Assay or Binding Assay Models

Size:

10 μg

100 μg

Price:

$ 660

CAT.:

P1HI0043S

Delivery:

2-3 weeks

Price:

$ 3433

CAT.:

P1HI0043L

Delivery:

2-3 weeks

KeyTec® EPHA4, N-GST

10 μg

100 μg

CAT.: P1HI0043S

$ 660 $ 3433

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® EPHA4, N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity and followed by SEC chromatography. The SEC-HPLC result showed that the EPHA4 protein was trimer in the solution. The EPHA4 protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P54764, E586-V986
Tag： N-terminal GST tag
Molecular Weight： 71.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 1.10 mg/mL by OD280
Purity：>90% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 20mM Reduced Glu, 0.05% Brij-35, 1mM DTT, 10% glycerol, pH7.5

Performance

The EPHA4 activity was detected using TR-FRET technology. The reaction was performed by incubating the EPHA4 protein, ATP and substrate at 25 ℃ for 50 min, adding the TR-FRET reagents at 25℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.