KeyTec® FGFR2[C491F], N-GST

Quick replication of product information

Used for Developing FGFR2[C491F] Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing FGFR2[C491F] Biochemical Activity Assay or Binding Assay Models

Size:

10 μg

100 μg

Price:

$ 660

CAT.:

P1HI0133S

Delivery:

2-3 weeks

Price:

$ 3990

CAT.:

P1HI0133L

Delivery:

2-3 weeks

KeyTec® FGFR2[C491F], N-GST

10 μg

100 μg

CAT.: P1HI0133S

$ 660 $ 3990

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® FGFR2 [C491F], N-GST recombinant protein with N-terminal GST tag + TEV cleavage site was purified by GST affinity and followed SEC chromatography. The SEC-HPLC result showed that the FGFR2 [C491F] was tetramer in the solution. The FGFR2 [C491F] protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P21802-1, R399-T821, C491F
Tag： N-terminal GST tag
Molecular Weight： 74.7 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.71 mg/mL by OD280
Purity：>95% by SDS-PAGE
Form： Liquid
Formulation： 50 mM Tris, 150 mM NaCl, 1mM DTT, 10% glycerol, 0.05% Brij35, pH7.5

Performance

The FGFR2 [C491F] activity was detected using TR-FRET technology. The reaction was performed by incubating the FGFR2 [C491F] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.