KeyTec® Firefly-Renilla Luciferase Detection Kit

Quick replication of product information

Luminescent Assay for Detecting Firefly and Renilla Luciferase in dual Reporter Gene Assays

• Easy to Use: A homogeneous assay - detect firefly and Renilla luciferase activities in one well
• High Sensitivity: Wider linear range and robust luminescence signal (with a half-life of approximately 1-2 h)
• The normalization can control for specific or nonspecific cellular responses and transfection efficiencies

Luminescent Assay for Detecting Firefly and Renilla Luciferase in dual Reporter Gene Assays

Size:

10 mL

2*50 mL

2*250 mL

Price:

$ 186

CAT.:

A2000900N

Delivery:

Available in Stock

Price:

$ 1173

CAT.:

A2000901N

Delivery:

Available in Stock

Price:

$ 4348

CAT.:

A2000902N

Delivery:

Available in Stock

KeyTec® Firefly-Renilla Luciferase Detection Kit

10 mL

2*50 mL

2*250 mL

CAT.: A2000900N

$ 186 $ 1173 $ 4348

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® Firefly-Renilla luciferase Detection kit is designed for the sensitive and robust detection of Firefly-Renilla dual luciferase reporter gene assays. The Firefly reagent detects Firefly luciferase activities, enabling accurate measurement of genetic element regulation. Additionally, the Renilla reagent quenches the Firefly luminescent signal and detects Renilla luciferase activities for normalization. This normalization can control for specific or nonspecific cellular responses and transfection efficiencies.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with Firefly luciferase, generating a sensitive luminescence signal with a half-life of approximately 2 hours. Addition of the KeyTec® Renilla Luciferase Stop & reaction buffer quenches the Firefly reaction's luminescence, and simultaneously, Coelenterazine reacts with Renilla luciferase, generating a luminescence signal that can also be read within 2 hours. The process provides a highly sensitive, robust, and homogeneous assay for the detection of Firefly-Renilla luciferase reporter gene expression in mammalian cells.

Performance

Use KeyTec® Firefly-Renilla Luciferase Detection Kit to detect the overexpression of Firefly luciferase and Renilla luciferase in the HEK293 cell line. The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Ten minutes after adding the Firefly luciferase detection reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s). Then ten minutes after adding the Renilla luciferase detection reagent, measure the luminescence signal using the Envision's Luminescence program.

Protocols

A2000902N_KeyTec® Firefly-Renilla Luciferase Detection Kit_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A2000900N	KeyTec® Firefly-Renilla Luciferase Detection Kit	10 mL
Firefly detection kit contains sufficient reagents to perform 100 tests of 75 μL/well. Renilla detection kit contains sufficient reagents to perform 100 tests of 75 μL/well. The kit contains the following components: • 1 × 100 μL KeyTec® Firefly Luciferase Substrate 100X • 1 × 10 mL KeyTec® Firefly Luciferase Cell lysis buffer • 1 × 100 μL KeyTec® Renilla Luciferase Substrate 100X • 1 × 10 mL KeyTec® Renilla Luciferase Stop & reaction buffer

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-40 ℃

Overview

Performance

Protocols

A2000902N_KeyTec® Firefly-Renilla Luciferase Detection Kit_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A2000901N	KeyTec® Firefly-Renilla Luciferase Detection Kit	2*50 mL
Firefly detection kit contains sufficient reagents to perform 1,000 tests of 75 μL/well. Renilla detection kit contains sufficient reagents to perform 1,000 tests of 75 μL/well. The kit contains the following components: • 2 × 500 μL KeyTec® Firefly Luciferase Substrate 100X • 2 × 50 mL KeyTec® Firefly Luciferase Cell lysis buffer • 2 × 500 μL KeyTec® Renilla Luciferase Substrate 100X • 2 × 50 mL KeyTec® Renilla Luciferase Stop & reaction buffer

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-40 ℃

Overview

Performance

Protocols

A2000902N_KeyTec® Firefly-Renilla Luciferase Detection Kit_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A2000902N	KeyTec® Firefly-Renilla Luciferase Detection Kit	2*250 mL
Firefly detection kit contains sufficient reagents to perform 5,000 tests of 75 μL/well. Renilla detection kit contains sufficient reagents to perform 5,000 tests of 75 μL/well. The kit contains the following components: • 4 × 1.25 mL KeyTec® Firefly Luciferase Substrate 100X • 2 × 250 mL KeyTec® Firefly Luciferase Cell lysis buffer • 4 × 1.25 mL KeyTec® Renilla Luciferase Substrate 100X • 2 × 250 mL KeyTec® Renilla Luciferase Stop & reaction buffer