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KeyTec® FLT3-ITD[F691L], N-GST; C-His

Quick replication of product information

KeyTec® FLT3-ITD[F691L], N-GST; C-His

Size: Used for Developing FLT3-ITD[F691L] Biochemical Activity Assay or Binding Assay Models

Price: $ 660

CAT.: P1HI0147S

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Used for Developing FLT3-ITD[F691L] Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing FLT3-ITD[F691L] Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
Price:
$ 660
CAT.:
P1HI0147S
Delivery:
2-3 weeks
Price:
$ 5291
CAT.:
P1HI0147L
Delivery:
2-3 weeks
KeyTec® FLT3-ITD[F691L], N-GST; C-His
10 μg
10 μg
100 μg
CAT.: P1HI0147S
$ 660 $ 5291
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® FLT3-ITD[F691L], N-GST; C-His recombinant protein with N-terminal GST tag + 3C cleavage site and C-terminal 6xHis tag was purified by Ni-NTA affinity chromatography. The FLT3-ITD[F691L] protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: P36888-1, R571-S993, F691L
Tag： N-terminal GST tag, C-terminal 6x His tag
Molecular Weight： 77.7 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.10 mg/mL by OD280
Purity：>95% by SDS-PAGE
Form：Liquid
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5

Performance

P1HI0147_2.1.png
P1HI0147_2.2.png
The FLT3-ITD[F691L] activity was detected using TR-FRET technology. The reaction was performed by incubating the FLT3-ITD[F691L] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0147_2.3.png

Protocols

COA_P1HI0147_K130824012_EN.pdf
COA_P1HI0147_K130824012_EN.pdf Download

Components

CAT. Description Size
P1HI0147S KeyTec® FLT3-ITD[F691L], N-GST; C-His 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® FLT3-ITD[F691L], N-GST; C-His recombinant protein with N-terminal GST tag + 3C cleavage site and C-terminal 6xHis tag was purified by Ni-NTA affinity chromatography. The FLT3-ITD[F691L] protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: P36888-1, R571-S993, F691L
Tag： N-terminal GST tag, C-terminal 6x His tag
Molecular Weight： 77.7 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.10 mg/mL by OD280
Purity：>95% by SDS-PAGE
Form：Liquid
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5

Performance

P1HI0147_2.1.png
P1HI0147_2.2.png
The FLT3-ITD[F691L] activity was detected using TR-FRET technology. The reaction was performed by incubating the FLT3-ITD[F691L] protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0147_2.3.png

Protocols

COA_P1HI0147_K130824012_EN.pdf
COA_P1HI0147_K130824012_EN.pdf Download

Components

CAT. Description Size
P1HI0147L KeyTec® FLT3-ITD[F691L], N-GST; C-His 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

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