KeyTec® GAK, N-His

Quick replication of product information

Used for Developing GAK Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing GAK Biochemical Activity Assay or Binding Assay Models

Size:

10 μg

100 μg

Price:

$ 660

CAT.:

P1HE0063S

Delivery:

2-3 weeks

Price:

$ 3990

CAT.:

P1HE0063L

Delivery:

2-3 weeks

KeyTec® GAK, N-His

10 μg

100 μg

CAT.: P1HE0063S

$ 660 $ 3990

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® GAK, N-His recombinant protein with N-terminal 6xHis tag was purified by Ni-NTA affinity and followed by SEC chromatography. The SEC-HPLC result showed that the GAK protein was monomer in the solution. The GAK protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: O14976;A12-P347
Tag： N-terminal 6xHis tag
Molecular Weight： 39.8 kDa
Species： Human
Expression Host： E.coli
Protein Concentration： 0.88 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Tris, 200 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5

Performance

The GAK activity was detected using ADP-Glo technology. The reaction was performed by incubating the GAK protein, ATP and substrate at 25 ℃ for 120 min, then reading RLU by Microplate Reader with Luminescence.