KeyTec® IL23A/IL12B, C-His/No tag

Quick replication of product information

Used for Developing IL23A/IL12B Biochemical Activity Assay or Binding Assay Models

• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing IL23A/IL12B Biochemical Activity Assay or Binding Assay Models

Size:

10 μg

100 μg

Price:

$ 334

CAT.:

P6HH0038S

Delivery:

2-3 weeks

Price:

$ 2002

CAT.:

P6HH0038L

Delivery:

2-3 weeks

KeyTec® IL23A/IL12B, C-His/No tag

10 μg

100 μg

CAT.: P6HH0038S

$ 334 $ 2002

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® IL23A/IL12B, C-His/No tag recombinant protein was co-expressed recombinant human IL23A with C-terminal 6x His tag, IL12B protein. Recombinant human IL23A/IL12B protein was purified by Ni-NTA affinity. SEC-HPLC result showed that the IL23A/IL12B protein was a monomer in the solution. The IL23A/IL12B protein showed high activity in TR-FRET assay.
AA Sequences：Uniprot: IL23A: Q9NPF7, R20-P189(end); IL12B: P29460, I23-S328(end)
Tag： IL23A: C-terminal 6x His tag; IL12B: No tag
Molecular Weight： IL23A: 19.6 kDa; IL12B: 34.7 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.87 mg/mL by OD280
Purity：>90% by SDS-PAGE
Form： Liquid
Formulation： PBS, pH7.5

Performance

The IL23A/IL12B activity was detected using TR-FRET technology. The reaction was performed by incubating the IL23A/IL12B protein, IL23R and the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.