(+86)-021-5835-6939 marketing@vkeybio.com
EnglishEnglish
Shanghai VKEY Biotechnologies Co.,Ltd.
Shanghai VKEY Biotechnologies Co.,Ltd.
Email Us

KeyTec® KIT, N-GST

Quick replication of product information

KeyTec® KIT, N-GST

Size: Used for Developing KIT Biochemical Activity Assay or Binding Assay Models

Price: $ 660

CAT.: P1HI0154S

激酶_1.1.jpg
激酶_1.2.jpg
激酶_1.1.jpg
激酶_1.1.jpg
激酶_1.1.jpg
激酶_1.2.jpg
激酶_1.1.jpg
激酶_1.1.jpg
Used for Developing KIT Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing KIT Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
Price:
$ 660
CAT.:
P1HI0154S
Delivery:
2-3 weeks
Price:
$ 3433
CAT.:
P1HI0154L
Delivery:
2-3 weeks
KeyTec® KIT, N-GST
10 μg
10 μg
100 μg
CAT.: P1HI0154S
$ 660 $ 3433
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® KIT, N-GST recombinant protein with N-terminal GST tag was purified by GST affinity chromatography. The KIT protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P10721-1, T544-V976
Tag： N-terminal GST tag
Molecular Weight： 74.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.61 mg/mL by OD280
Purity：>90% by SDS-PAGE
Form：Liquid
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5

Performance

P1HI0154_2.1.png
P1HI0154_2.2.png
The KIT activity was detected using TR-FRET technology. The reaction was performed by incubating the KIT protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0154_2.3.png

Protocols

COA_P1HI0154_K130901012_EN.pdf
COA_P1HI0154_K130901012_EN.pdf Download

Components

CAT. Description Size
P1HI0154S KeyTec® KIT, N-GST 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® KIT, N-GST recombinant protein with N-terminal GST tag was purified by GST affinity chromatography. The KIT protein showed high activity in TR-FRET assay.
AA Sequences： Uniprot: P10721-1, T544-V976
Tag： N-terminal GST tag
Molecular Weight： 74.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.61 mg/mL by OD280
Purity：>90% by SDS-PAGE
Form：Liquid
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, 0.05% Brij35, pH7.5

Performance

P1HI0154_2.1.png
P1HI0154_2.2.png
The KIT activity was detected using TR-FRET technology. The reaction was performed by incubating the KIT protein, ATP and substrate at 25 ℃ for 60 min, adding the TR-FRET reagents at 25 ℃ for 60 min, then reading ratio 665/620 by Microplate Reader with TR-FRET.
P1HI0154_2.3.png

Protocols

COA_P1HI0154_K130901012_EN.pdf
COA_P1HI0154_K130901012_EN.pdf Download

Components

CAT. Description Size
P1HI0154L KeyTec® KIT, N-GST 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Related Kinase

KeyTec® DDR2, N-GST KeyTec® DDR2, N-GST
KeyTec® PKCθ, N-GST;C-Flag KeyTec® PKCθ, N-GST;C-Flag
KeyTec® Biotin-PIK3CA[H1047R]/PIK3R1, C-His/N-Flag; C-Avi KeyTec® Biotin-PIK3CA[H1047R]/PIK3R1, C-His/N-Flag; C-Avi
KeyTec® ERN2, N-GST; C-StrepII KeyTec® ERN2, N-GST; C-StrepII
KeyTec® IRAK4, N-GST; C-StrepII KeyTec® IRAK4, N-GST; C-StrepII
KeyTec® CDK12/Cyclin K, N-GST KeyTec® CDK12/Cyclin K, N-GST
KeyTec® Biotin-Nuak2, N-Flag;C-Avi KeyTec® Biotin-Nuak2, N-Flag;C-Avi
KeyTec® HIPK3, N-GST;C-Flag KeyTec® HIPK3, N-GST;C-Flag