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Shanghai VKEY Biotechnologies Co.,Ltd.
Shanghai VKEY Biotechnologies Co.,Ltd.
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KeyTec® MAP2K1, N-His

Quick replication of product information

KeyTec® MAP2K1, N-His

Size: Used for Developing MAP2K1 Biochemical Activity Assay or Binding Assay Models

Price: $ 660

CAT.: P1HI0071S

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Used for Developing MAP2K1 Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing MAP2K1 Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
Price:
$ 660
CAT.:
P1HI0071S
Delivery:
2-3 weeks
Price:
$ 3433
CAT.:
P1HI0071L
Delivery:
2-3 weeks
KeyTec® MAP2K1, N-His
10 μg
10 μg
100 μg
CAT.: P1HI0071S
$ 660 $ 3433
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® MAP2K1, N-His recombinant protein with N-terminal 8xHis tag + TEV clevage site was purified by Ni-NTA affinity chromatography. The MAP2K1 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: Q02750-1, M1-V393(end)
Tag： N-terminal 8xHis tag
Molecular Weight： 45.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.74 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Hepes, 300 mM NaCl, 1 mM DTT, 0.05% Brij-35, 10% glycerol, pH7.5

Performance

P1HI0071_2.1.png
P1HI0071_2.2.png
The MAP2K1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the MAP2K1 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
P1HI0071_2.3.png

Protocols

COA_P1HI0071_K130425011_EN.pdf
COA_P1HI0071_K130425011_EN.pdf Download

Components

CAT. Description Size
P1HI0071S KeyTec® MAP2K1, N-His 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® MAP2K1, N-His recombinant protein with N-terminal 8xHis tag + TEV clevage site was purified by Ni-NTA affinity chromatography. The MAP2K1 protein showed high activity in ADP-Glo assay.
AA Sequences： Uniprot: Q02750-1, M1-V393(end)
Tag： N-terminal 8xHis tag
Molecular Weight： 45.9 kDa
Species： Human
Expression Host： Sf9
Protein Concentration： 0.74 mg/mL by OD280
Purity：> 95% by SDS-PAGE
Form： Liquid
Formulation： 20 mM Hepes, 300 mM NaCl, 1 mM DTT, 0.05% Brij-35, 10% glycerol, pH7.5

Performance

P1HI0071_2.1.png
P1HI0071_2.2.png
The MAP2K1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the MAP2K1 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
P1HI0071_2.3.png

Protocols

COA_P1HI0071_K130425011_EN.pdf
COA_P1HI0071_K130425011_EN.pdf Download

Components

CAT. Description Size
P1HI0071L KeyTec® MAP2K1, N-His 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

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