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KeyTec® PIK3CA/PIK3R1, C-His/N-Flag; C-Avi

Quick replication of product information

KeyTec® PIK3CA/PIK3R1, C-His/N-Flag; C-Avi

Size: Used for Developing PIK3CA/PIK3R1 Biochemical Activity Assay or Binding Assay Models

Price: $ 946

CAT.: P1HI0161S

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Used for Developing PIK3CA/PIK3R1 Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Used for Developing PIK3CA/PIK3R1 Biochemical Activity Assay or Binding Assay Models


• Strict quality control: Each batch comes with a rigorous QC report
• High activity: Each batch is activity-verified, providing high-quality protein
• Validated with homogeneous assay models, such as TR-FRET and ADP-Glo, ideal for high-throughput screening

Size:
10 μg
100 μg
Price:
$ 946
CAT.:
P1HI0161S
Delivery:
2-3 weeks
Price:
$ 5291
CAT.:
P1HI0161L
Delivery:
2-3 weeks
KeyTec® PIK3CA/PIK3R1, C-His/N-Flag; C-Avi
10 μg
10 μg
100 μg
CAT.: P1HI0161S
$ 946 $ 5291
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® PIK3CA/PIK3R1, C-His/N-Flag; C-Avi recombinant protein was co-expressed recombinant human PIK3CA with C-terminal 8xHis tag and PIK3R1 with N-terminal Flag tag and C-terminal Avi tag. The complex was purified by Ni-NTA affinity chromatography. The PIK3CA/PIK3R1 protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: PIK3CA: P42336, M1-N1068(end); PIK3R1: P27986-1, M1-R724(end)
Tag： PIK3CA: C-terminal 8x His tag; PIK3R1: N-terminal Flag tag, C-terminal Avi tag
Molecular Weight： PIK3CA: 125.5 kDa; PIK3R1: 86.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.67 mg/mL by OD280
Purity：>95% by SDS-PAGE
Form：Liquid
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5

Performance

P1HI0161_2.1.png
The PIK3CA/PIK3R1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the PIK3CA/PIK3R1 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
P1HI0161_2.3.png

Protocols

COA_P1HI0161_K140109011_EN.pdf
COA_P1HI0161_K140109011_EN.pdf Download

Components

CAT. Description Size
P1HI0161S KeyTec® PIK3CA/PIK3R1, C-His/N-Flag; C-Avi 10 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

Overview

KeyTec® PIK3CA/PIK3R1, C-His/N-Flag; C-Avi recombinant protein was co-expressed recombinant human PIK3CA with C-terminal 8xHis tag and PIK3R1 with N-terminal Flag tag and C-terminal Avi tag. The complex was purified by Ni-NTA affinity chromatography. The PIK3CA/PIK3R1 protein showed high activity in ADP-Glo assay.
AA Sequences：Uniprot: PIK3CA: P42336, M1-N1068(end); PIK3R1: P27986-1, M1-R724(end)
Tag： PIK3CA: C-terminal 8x His tag; PIK3R1: N-terminal Flag tag, C-terminal Avi tag
Molecular Weight： PIK3CA: 125.5 kDa; PIK3R1: 86.8 kDa
Species：Human
Expression Host：Sf9
Protein Concentration：0.67 mg/mL by OD280
Purity：>95% by SDS-PAGE
Form：Liquid
Formulation：50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH7.5

Performance

P1HI0161_2.1.png
The PIK3CA/PIK3R1 activity was detected using ADP-Glo technology. The reaction was performed by incubating the PIK3CA/PIK3R1 protein, ATP and substrate at 25 ℃ for 60 min, then reading RLU by Microplate Reader with Luminescence.
P1HI0161_2.3.png

Protocols

COA_P1HI0161_K140109011_EN.pdf
COA_P1HI0161_K140109011_EN.pdf Download

Components

CAT. Description Size
P1HI0161L KeyTec® PIK3CA/PIK3R1, C-His/N-Flag; C-Avi 100 μg

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -80 ℃

-80 ℃

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