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KeyTec® Renilla Luciferase Detection Kit

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KeyTec® Renilla Luciferase Detection Kit

Size: Luminescent Assay for Detecting Renilla Luciferase in Reporter Gene Assays

Price: $ 119

CAT.: A2001100N

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Luminescent Assay for Detecting Renilla Luciferase in Reporter Gene Assays


• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: Wider linear range and low background
• No need for separate lysis, and the luminescence signal is not only high but also robust, with a half-life of approximately 1-2 h

Luminescent Assay for Detecting Renilla Luciferase in Reporter Gene Assays


• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: Wider linear range and low background
• No need for separate lysis, and the luminescence signal is not only high but also robust, with a half-life of approximately 1-2 h

Luminescent Assay for Detecting Renilla Luciferase in Reporter Gene Assays


• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: Wider linear range and low background
• No need for separate lysis, and the luminescence signal is not only high but also robust, with a half-life of approximately 1-2 h

Size:
10 mL
2*50 mL
2*250 mL
Price:
$ 119
CAT.:
A2001100N
Delivery:
Available in Stock
Price:
$ 645
CAT.:
A2001101N
Delivery:
Available in Stock
Price:
$ 3113
CAT.:
A2001102N
Delivery:
Available in Stock
KeyTec® Renilla Luciferase Detection Kit
10 mL
10 mL
2*50 mL
2*250 mL
CAT.: A2001100N
$ 119 $ 645 $ 3113
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

KeyTec® Renilla luciferase Detection kit is designed for the sensitive and robust detection of Renilla luciferase reporter gene assays. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Renilla luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, Coelenterazine reacts with Renilla luciferase released by cells, generating a sensitive and robust luminescence signal. The process provides a highly sensitive, robust, and homogeneous assay for the detection of Renilla luciferase reporter gene expression in mammalian cells.
EN_A2001100N_2.1.png

Performance

Use KeyTec® Renilla Luciferase Detection Kit to detect the overexpression of Renilla luciferase in the HEK293 cell line . The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
A2001100N_3.1.png

Protocols

Components

CAT. Description Size
A2001100N KeyTec® Renilla Luciferase Detection Kit 10 mL
Each kit contains sufficient reagents to perform 100 tests of 100 μL/well.
The kit contains the following components:
• 1 × 100 μL KeyTec® Renilla Luciferase Substrate 100X
• 1 × 10 mL KeyTec® Renilla Luciferase Cell lysis buffer

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -20 ℃

-20 ℃

Overview

KeyTec® Renilla luciferase Detection kit is designed for the sensitive and robust detection of Renilla luciferase reporter gene assays. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Renilla luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, Coelenterazine reacts with Renilla luciferase released by cells, generating a sensitive and robust luminescence signal. The process provides a highly sensitive, robust, and homogeneous assay for the detection of Renilla luciferase reporter gene expression in mammalian cells.
EN_A2001100N_2.1.png

Performance

Use KeyTec® Renilla Luciferase Detection Kit to detect the overexpression of Renilla luciferase in the HEK293 cell line . The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
A2001100N_3.1.png

Protocols

Components

CAT. Description Size
A2001101N KeyTec® Renilla Luciferase Detection Kit 2*50 mL
Each kit contains sufficient reagents to perform 1,000 tests of 100 μL/well.
The kit contains the following components:
• 2 × 500 μL KeyTec® Renilla Luciferase Substrate 100X
• 2 × 50 mL KeyTec® Renilla Luciferase Cell lysis buffer

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -20 ℃

-20 ℃

Overview

KeyTec® Renilla luciferase Detection kit is designed for the sensitive and robust detection of Renilla luciferase reporter gene assays. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Renilla luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, Coelenterazine reacts with Renilla luciferase released by cells, generating a sensitive and robust luminescence signal. The process provides a highly sensitive, robust, and homogeneous assay for the detection of Renilla luciferase reporter gene expression in mammalian cells.
EN_A2001100N_2.1.png

Performance

Use KeyTec® Renilla Luciferase Detection Kit to detect the overexpression of Renilla luciferase in the HEK293 cell line . The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).
A2001100N_3.1.png

Protocols

Components

CAT. Description Size
A2001102N KeyTec® Renilla Luciferase Detection Kit 2*250 mL
Each kit contains sufficient reagents to perform 5,000 tests of 100 μL/well.
The kit contains the following components:
• 4 × 1.25 mL KeyTec® Renilla Luciferase Substrate 100X
• 2 × 250 mL KeyTec® Renilla Luciferase Cell lysis buffer

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -20 ℃

-20 ℃

Related Reporter Assays