KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit

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TR-FRET Assay for measurement of the interaction between KRAS[G12D] and RAF1 proteins

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Accuracy: Utilizes GDP-loaded KRAS[G12D] protein, aligning more closely with physiological conditions.
• High S/B: The kit provides a superior window

TR-FRET Assay for measurement of the interaction between KRAS[G12D] and RAF1 proteins

Size:

1,000 tests

10,000 tests

Price:

$ 2000

CAT.:

A1090002S

Delivery:

Available in Stock

Price:

$ 12000

CAT.:

A1090002L

Delivery:

Available in Stock

KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit

1,000 tests

10,000 tests

CAT.: A1090002S

$ 2000 $ 12000

Overview

Performance

Protocols

Components

Notices

Overview

The KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit is designed for measurement of the interaction between KRAS[G12D] and RAF1 proteins. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, high accuracy.
The detection principle is based on TR-FRET technology. The Tag2-RAF1 protein binds to the donor (KeyTec® TR-FRET mAb anti-HIS-Solar Eu), and the Tag1-KRAS[G12D] [GDP loaded] protein binds to the acceptor (mAb anti-Tag1–LA). When KRAS[G12D] [GDP loaded] interacts with RAF1, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction. Blocking this interaction with any compound, peptide, or antibody will reduce the TR-FRET signal.

Performance

•The inhibitory effects of small molecules

•The results of GTP EC50

•The results of GDP IC50

Protocols

Protocol_A1090002_KeyTec® TR-FRET GDP loaded KRAS[G12D] ∕ RAF1 Binding Assay kit_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A1090002S	KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit	1,000 tests
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. • 1 × 45 µL Tag1-KRAS[G12D] [ GDP loaded ] (100X) • 1 × 45 µL Tag2-RAF1 (100X) • 1 × 50 µL mAb anti-Tag2–Solar Eu (100X) • 1 × 50 µL mAb anti-Tag1–LA (100X) • 1 × 45 µL GTP (100X，2.5 mM) • 1 × 50 mL Binding Assay Diluent Buffer • 1 × 30 mL Solar Eu Detection Buffer

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-60℃ or below

Overview

Performance

•The inhibitory effects of small molecules

•The results of GTP EC50

•The results of GDP IC50

Protocols

Protocol_A1090002_KeyTec® TR-FRET GDP loaded KRAS[G12D] ∕ RAF1 Binding Assay kit_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A1090002L	KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit	10,000 tests
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. • 1 × 410 µL Tag1-KRAS[G12D] [ GDP loaded ] (100X) • 1 × 410 µL Tag2-RAF1 (100X) • 1 × 500 µL mAb anti-Tag2–Solar Eu (100X) • 1 × 500 µL mAb anti-Tag1–LA (100X) • 1 × 410 µL GTP (100X，2.5 mM) • 1 × 200 mL Binding Assay Diluent Buffer • 1 × 120 mL Solar Eu Detection Buffer

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-60℃ or below

KeyTec® TR-FRET GDP loaded KRAS[G12D]/RAF1 Binding Assay kit

Overview

Performance

Protocols

Components

Notices

Overview

Performance

Protocols

Components

Notices

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