KeyTec® TR-FRET Human Fc Detection kit

Quick replication of product information

Size:

1,000 tests

10,000 tests

Price:

$ 1484

CAT.:

A1040002S

Delivery:

Available in Stock

Price:

$ 5100

CAT.:

A1040002L

Delivery:

Available in Stock

KeyTec® TR-FRET Human Fc Detection kit

1,000 tests

10,000 tests

CAT.: A1040002S

$ 1484 $ 5100

Overview

Performance

Protocols

Components

Notices

Overview

The KeyTec® TR-FRET Human Fc detection kit is designed for quantitative measurement of human IgG or human Fc-tagged protein in supernatant. It is based on competitive immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, wide range, robustness.
The screening principle is based on TR-FRET technology. The mAb anti-hFc antibody is labeled with KeyTec® TR-FRET Solar Eu and Human IgG is labeled with KeyTec® TR-FRET LA, when mAb anti-hFc-Solar Eu binds to Human IgG-LA, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm. Native human IgG or human Fc-tagged protein competes with the Human IgG-LA for binding to anti-hFc antibodies. The specific signal is inversely proportional to the concentration of human IgG or human Fc-tagged protein in the standard or sample.

Performance

• Standard Curve
• Quantitative Range：50 ng/mL – 50, 000 ng/mL
• Incubation Condition：Incubate at room temperature for 2 hours

Protocols

Protocol_A1040002_KeyTec® TR-FRET Human Fc Detection kit_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A1040002S	KeyTec® TR-FRET Human Fc Detection kit	1,000 tests
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. • 1 × 100 µL mAb anti-hFc - Solar Eu (50X) • 1 × 100 µL mAb Human IgG-LA (50X) • 1 × 150 µL Human IgG Standard (5 µg/mL) • 1 × 30 mL Biotherapeutics Diluent Buffer • 1 × 10 mL Biotherapeutics Detection Buffer

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-60℃ or below

Overview

Performance

• Standard Curve
• Quantitative Range：50 ng/mL – 50, 000 ng/mL
• Incubation Condition：Incubate at room temperature for 2 hours

Protocols

Protocol_A1040002_KeyTec® TR-FRET Human Fc Detection kit_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A1040002L	KeyTec® TR-FRET Human Fc Detection kit	10,000 tests
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well. • 1 × 1,000 µL mAb anti-hFc - Solar Eu (50X) • 1 × 1,000 µL mAb Human IgG-LA (50X) • 2 × 150 µL Human IgG Standard (5 µg/mL) • 1 × 200 mL Biotherapeutics Diluent Buffer • 1 × 100 mL Biotherapeutics Detection Buffer