Shanghai VKEY Biotechnologies Co.,Ltd.
Shanghai VKEY Biotechnologies Co.,Ltd.
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KeyTec® TR-FRET Human TNFa Detection kit

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KeyTec® TR-FRET Human TNFa Detection kit

Size: Sandwich Model TR-FRET Assay for Quantitative Measurement of Human TNFα Protein in Supernatant

Price: $ 2102

CAT.: A1070001S

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Sandwich Model TR-FRET Assay for Quantitative Measurement of Human TNFα Protein in Supernatant


• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: A Wide linear range from 30 pg/mL to 5,000 pg/mL
• Only 16 µL of the sample, no dilution required, highly suitable for HTS Assays

Sandwich Model TR-FRET Assay for Quantitative Measurement of Human TNFα Protein in Supernatant


• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• High Sensitivity: A Wide linear range from 30 pg/mL to 5,000 pg/mL
• Only 16 µL of the sample, no dilution required, highly suitable for HTS Assays

Size:
1,000 tests
10,000 tests
Price:
$ 2102
CAT.:
A1070001S
Delivery:
Available in Stock
Price:
$ 9800
CAT.:
A1070001L
Delivery:
Available in Stock
KeyTec® TR-FRET Human TNFa Detection kit
1,000 tests
1,000 tests
10,000 tests
CAT.: A1070001S
$ 2102 $ 9800
Overview
Performance
Protocols
Components
Notices
Related Products

Overview

The KeyTec® TR-FRET Human TNFαassay kit is designed for quantitative measurement of Human TNFα protein in supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.
The detection principle is based on TR-FRET technology. A pair of mAb anti-TNFα antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, bind to the same antigen Human TNFα protein. This binding brings the donor molecule into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the concentration of Human TNFα protein.
EN_Cytokine_2.png

Performance

A1070001_3.1.png
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• performance
Quantitative Range:30 pg/mL – 5,000 pg/mL
Incubation Condition: Incubate at room temperature for 3 hours

Protocols

Components

CAT. Description Size
A1070001S KeyTec® TR-FRET Human TNFa Detection kit 1,000 tests
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
• 1 × 50 µL mAb anti-TNFα-Solar Eu (40X)
• 1 × 50 µL mAb anti-TNFα-LA (40X)
• 2 vials Lyophilized Human TNFα Standard
• 1 × 20 mL Cytokine Diluent Buffer
• 1 × 10 mL Cytokine Detection Buffer

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -60℃ or below

-60℃ or below

Overview

The KeyTec® TR-FRET Human TNFαassay kit is designed for quantitative measurement of Human TNFα protein in supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.
The detection principle is based on TR-FRET technology. A pair of mAb anti-TNFα antibodies, each labeled with KeyTec® TR-FRET Solar Eu and KeyTec® TR-FRET LA, bind to the same antigen Human TNFα protein. This binding brings the donor molecule into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the concentration of Human TNFα protein.
EN_Cytokine_2.png

Performance

A1070001_3.1.png
A1070001_3.2.png
• performance
Quantitative Range:30 pg/mL – 5,000 pg/mL
Incubation Condition: Incubate at room temperature for 3 hours

Protocols

Components

CAT. Description Size
A1070001L KeyTec® TR-FRET Human TNFa Detection kit 10,000 tests
The tests are sufficient in a 384-well microplate assay format, with 20 μL per well.
• 1 × 500 µL mAb anti-TNFα-Solar Eu (40X)
• 1 × 500 µL mAb anti-TNFα-LA (40X)
• 2 vials Lyophilized Human TNFα Standard
• 1 × 50 mL Cytokine Diluent Buffer
• 1 × 50 mL Cytokine Detection Buffer

Notices

Certificate of Analysis Limitations Storage Conditions
LOT. For research use only -60℃ or below

-60℃ or below

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