KeyTec® TR-FRET Hybridoma screening kit (His antigen, Mouse IgG)

Quick replication of product information

Size:

1,000 tests

10,000 tests

Price:

$ 1824

CAT.:

A1040003S

Delivery:

Available in Stock

Price:

$ 12100

CAT.:

A1040003L

Delivery:

Available in Stock

KeyTec® TR-FRET Hybridoma screening kit (His antigen, Mouse IgG)

1,000 tests

10,000 tests

CAT.: A1040003S

$ 1824 $ 12100

Overview

Performance

Protocols

Components

Notices

Overview

The KeyTec® TR-FRET Hybridoma screening kit (His antigen, Mouse IgG) is designed for the simple and rapid screening of positive mouse hybridoma clones based on antigen-antibody affinity in cell supernatant. It is based on sandwich immunoassay model and utilizes TR-FRET technology, known for its ease of use, homogeneity (no wash), low background, high sensitivity, robustness.
The screening principle is based on TR-FRET technology. The mAb anti His-Solar Eu binds to the HIS-tagged antigen, while the pAb anti-Mouse Fc-LA binds to the mouse Fc of IgG antibodies expressed by mouse hybridoma. When the antigen-antibody interact, the donor molecule is brought into proximity with the acceptor molecule. Excitation of the donor will result in the generation of the TR-FRET signal at 665 nm, proportional to the extent of protein interaction.

Performance

• Optimization of Antigen and Antibody Concentration
• Range of Antibody concentration: 100 ng/mL – 80 µg/mL*5
• Incubation Condition：Incubate at room temperature for 2 hours to overnight

• Effects of various matrices

Protocols

Protocol_A1040003_KeyTec® TR-FRET Hybridoma screening kit (His antigen, Mouse IgG)_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A1040003S	KeyTec® TR-FRET Hybridoma screening kit (His antigen, Mouse IgG)	1,000 tests
The system accommodates 384-well microplates, and assay volumes can be adjusted proportionally to perform in 96- or 1536-well microplates. • 1 × 100 µL mAb anti His - Solar Eu (50X) • 1 × 100 µL pAb anti-Mouse Fc - LA (50X) • 1 × 40 mL Hybridoma Detection Buffer • 1 × Positive Control (1X),Lyophilized

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-60℃ or below

Overview

Performance

• Optimization of Antigen and Antibody Concentration
• Range of Antibody concentration: 100 ng/mL – 80 µg/mL*5
• Incubation Condition：Incubate at room temperature for 2 hours to overnight

• Effects of various matrices

Protocols

Protocol_A1040003_KeyTec® TR-FRET Hybridoma screening kit (His antigen, Mouse IgG)_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A1040003L	KeyTec® TR-FRET Hybridoma screening kit (His antigen, Mouse IgG)	10,000 tests
The system accommodates 384-well microplates, and assay volumes can be adjusted proportionally to perform in 96- or 1536-well microplates. • 1 × 1 mL mAb anti His - Solar Eu (50X) • 1 × 1 mL pAb anti-Mouse Fc - LA (50X) • 1 × 200 mL Hybridoma Detection Buffer • 2 × Positive Control (1X),Lyophilized