KeyTec® Ultra Luciferase Detection Kit

Quick replication of product information

Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
• Extremely sensitive: Wider linear range and extremely luminescence signal
• With a very high and a short half-life (approximately 30 minutes) luminescence signal, it's suitable for applications that demand high signal sensitivity

Luminescent Assay for Detecting Firefly Luciferase in Reporter Gene Assays

Size:

10 mL

2*50 mL

2*250 mL

4*250 mL

Price:

$ 104

CAT.:

A2000300N

Delivery:

Available in Stock

Price:

$ 618

CAT.:

A2000301N

Delivery:

Available in Stock

Price:

$ 2232

CAT.:

A2000302N

Delivery:

Available in Stock

Price:

$ 5131

CAT.:

A2000303N

Delivery:

Available in Stock

KeyTec® Ultra Luciferase Detection Kit

10 mL

2*50 mL

2*250 mL

4*250 mL

CAT.: A2000300N

$ 104 $ 618 $ 2232 $ 5131

Overview

Performance

Protocols

Components

Notices

Overview

KeyTec® Ultra luciferase Detection kit is designed for the highly sensitive detection of Firefly luciferase reporter gene assays. Simply mix the substrate with cell lysis buffer, add the mixture to the cells, and proceed to detect the Firefly luciferase signal within the cells.
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin reacts with Firefly luciferase released by cells, generating a highly sensitive luminescence signal. The process provides a highly sensitive, robust, and homogeneous assay for the detection of Firefly luciferase reporter gene expression in mammalian cells.

Performance

Use KeyTec® Ultra Luciferase Detection Kit to detect the overexpression of Firefly luciferase in the HEK293 cell line . The results showed a linear relationship between the luminescence signal and the number of cells. HEK293 cells, cultured in DMEM medium with 10% FBS, were serially diluted two-fold, starting from 50,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in “Assay Procedure”. Five minutes after adding the reagent, measure the luminescence signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).

Protocols

A2000303N_KeyTec® Ultra Luciferase Detection Kit_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A2000300N	KeyTec® Ultra Luciferase Detection Kit	10 mL
Each kit contains sufficient reagents to perform 100 tests of 100 μL/well. The kit contains the following components: • 1 × 200 μL KeyTec® Stable Luciferase Substrate 50X • 1 × 10 mL KeyTec® Stable Luciferase Cell lysis buffer

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-40 ℃

Overview

Performance

Protocols

A2000303N_KeyTec® Ultra Luciferase Detection Kit_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A2000301N	KeyTec® Ultra Luciferase Detection Kit	2*50 mL
Each kit contains sufficient reagents to perform 1,000 tests of 100 μL/well. The kit contains the following components: • 2 × 1 mL KeyTec® Stable Luciferase Substrate 50X • 2 × 50 mL KeyTec® Stable Luciferase Cell lysis buffer

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-40 ℃

Overview

Performance

Protocols

A2000303N_KeyTec® Ultra Luciferase Detection Kit_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A2000302N	KeyTec® Ultra Luciferase Detection Kit	2*250 mL
Each kit contains sufficient reagents to perform 5,000 tests of 100 μL/well. The kit contains the following components: • 5 × 2 mL KeyTec® Stable Luciferase Substrate 50X • 2 × 250 mL KeyTec® Stable Luciferase Cell lysis buffer

Notices

Certificate of Analysis	Limitations	Storage Conditions
LOT.	For research use only	-40 ℃

Overview

Performance

Protocols

A2000303N_KeyTec® Ultra Luciferase Detection Kit_VKEYBIO-01-2024.pdf Download

Components

CAT.	Description	Size
A2000303N	KeyTec® Ultra Luciferase Detection Kit	4*250 mL
Each kit contains sufficient reagents to perform 10,000 tests of 100 μL/well. The kit contains the following components: • 2 × A2000302N KeyTec® Stable Luciferase Detection Kit (5,000 tests)