Luciferase genes can be integrated into the cell chromosomes through processes such as stable selection, allowing for continuous and stable expression as the cells divide, migrate, and differentiate. The half-life of luciferase is about 3 hours, so only living cells can continuously express luciferase.
As a substrate for Firefly luciferase, D-luciferin is commonly used in the following assays:
(1) In vivo imaging: Firefly luciferase plays a crucial role in in vivo imaging techniques. By labeling this enzyme to specific cells (such as tumor cells or stem cells) or pathogens, researchers can observe the behavior of these cells or pathogens in living organisms in real-time and non-invasively.
(2) In vitro studies: It can be used to analyze ATP levels, assess reporter gene expression, and for various applications such as high-throughput screening and contamination detection.
There is no difference between the two; it is merely a naming difference by different companies, and their chemical structures are identical.
After D-luciferin is injected into the animal, it distributes quickly and easily disperses throughout the body.
D-luciferin can be administered in various ways, with common methods including intraperitoneal injection, subcutaneous injection, and intravenous injection. The kinetic curves vary with different administration routes. For most studies, intraperitoneal/subcutaneous injection reaches peak luminescent signal approximately 10-20 minutes after administration, while intravenous injection reaches peak signal in about 2-5 minutes.
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