Targeting life health research, especially the requirements of high-throughput drug screening, VKEY-BIO can provide a range of highly sensitive, stable, and cost-effective reagents.
Our main offerings include the Cell Viability Detection Kit, Reporter Gene Detection Kit, In Vivo Imaging Reagent, PPI Tool-Box Reagent, Biomarker Detection Kit, Hybridoma Screening Kit, Antibodies Concentration Detection Kit, Phosphoprotein Detection Kit, Kinase and Epigenetic enzyme, and Consumables.
Additionally, VKEY-BIO provides customized assay development services to cater to the specific needs of our customers.
VKEY-BIO provides homogenous assay format-related products significantly reduce assay time and minimize steps. With just add, incubate, and read, then you can get optimal results efficiently, saving time and ensuring accuracy results and reduce the error by operation.
VKEY-BIO provides high-performance products at competitive prices, ensuring that you can get optimal results while staying within budget constraints
VKEY-BIO maintains rigorous quality control standards throughout the manufacturing process, ensuring consistency, reliability, and reproducibility in every product batch, thereby enhancing the confidence of researchers in your assays.
Before initiating a TR-FRET assay, it's essential to verify that your microplate reader is equipped with a TRF module. If you're uncertain about your reader's compatibility, please send the reader type and brand to technical-support@vkeybio.com for further assistance.
The most common routes of administration for D-Luciferin potassium salt in small animal live imaging are intraperitoneal (IP) injection and tail vein injection. Each route has its own characteristics:
Intraperitoneal injection: Provides slow diffusion and onset of luminescence, reaching peak signal in approximately 10-20 minutes, but offers a longer duration of luminescence.
Tail vein injection: Offers faster diffusion and onset of luminescence, reaching peak signal in about 2-5 minutes, but provides a shorter duration of luminescence.
This ratio ensures that each of the four identical peptide chains of Streptavidin (SA) can bind to one biotin molecule. It's crucial to match the concentrations of SA-LA/HX and Europium (Eu) to avoid the "hook effect" caused by excessive Streptavidin, which could compromise the accuracy of the assay.
The Stimulation buffer is designed to resuspend cells and maintain their normal cellular activity within a short period (within 2 hours). When preparing it, it's important to add IBMX to a final concentration of 0.5 mM. It's recommended to prepare the Stimulation buffer immediately before use to ensure its effectiveness.
In a PPI model, the Binding Assay Diluent Buffer is primarily used for diluting protein samples, while the Solar Eu/Tb Detection Buffer is specifically optimized for detecting antibody dilutions.
Yes, the Cell Viability Assay Kit can be used to detect the viability of both adherent and suspension cells. The principle of the assay involves quantitatively measuring the ATP content of lysed cells to determine the number of metabolically active living cells
English
