The detection principle is based on Luminescent technology. Within the kit, D-Luciferin and luciferase react with ATP released by cells to generate a bright and stable luminescent signal. The ATP - based chemiluminescent cell viability assay kit has the features of simple operation, high sensitivity and good stability, which has become one of the gold - standard methods for the assessment of cell viability and drug toxicity , and is widely used in pharmaceutical companies at home and abroad.