Bioluminescence and fluorescence are the two main techniques used for in vivo optical imaging of living animals. Bioluminescence uses firefly luciferase produced by expression of a luciferase reporter gene in vivo to generate luminescence by chemical reaction with a luciferase substrate injected in vitro.
KeyTec® D - Luciferin potassium salt is an efficient, stable and reliable substrate for firefly luciferase, and is often used in in vitro live cell and in vivo bioluminescence imaging studies. It has excellent water and lipid solubility properties, enabling it to easily cross cell membranes and stably generate high - intensity luminescence signals.
The purity can reach more than 99%.
Maximum solubility in water up to 42 mg/mL.
It can penetrate cell membranes to provide the substrate required for luciferase reaction.
High-performance at competitive prices.
KeyTec® D - luciferin potassium salt penetrates the cell membrane and enters living cells, where it reacts with firefly luciferase to generate oxyluciferin and a luminescent phenomenon. Signals can be detected using a microplate reader or a chemiluminescence imaging instrument.
In vivo imaging technology can be used to monitor biological processes such as tumor growth and metastasis, inflammation, specific gene expression and drug efficacy in living animals.
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