Time-Resolved Fluorescence (TRF) Analysis: This method is based on the long fluorescence effect of lanthanide chelates and involves fluorescence analysis from both time and fluorescence intensity dimensions. Since its establishment in the 1980s, this technique has effectively eliminated the interference of non-specific fluorescence, significantly enhancing analytical sensitivity.
Eu3+/Sm3+ labeling reagents are used to label macromolecules like proteins, peptides, and oligonucleotides, and the resulting markers are suitable for various types of analysis based on solid phase separations. After a series of manipulation steps, the remaining Eu3+/Sm3+-containing marker is immobilized onto a microtiter plate after the unbound portion is washed away. This solution is then added and can dissociate Eu3+/Sm3+ from the immobilized labeled antibody or protein within a few minutes, forming a homogeneous time-resolved fluorescent micellar chelate solution with extremely high brightness. highly sensitive measurements of analytes can be performed.. It is compatible with DELFIA assay.
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